【文獻標題】Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply ａnd Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes
【作者】Longlong Li ,Mengling Peng ,Chongyang Ge, et.al
【作者單位】南京農(nóng)業(yè)大學（Nanjing Agricultural University）
【文獻中引用產(chǎn)品】
雞糖原磷酸化酶(GP)ELISA試劑盒
雞糖原合成酶(GS)ELISA試劑盒
雞葡萄糖激酶(GK)ELISA試劑盒
雞磷酸果糖激酶-1（PFK-1）ELISA試劑盒
雞丙酮酸激酶（PK）ELISA試劑盒
雞丙酮酸脫氫酶E1(PDH E1)ELISA試劑盒
雞檸檬酸合成酶(CS)ELISA試劑盒
雞烏頭酸酶(ACO)ELISA試劑盒
雞磷酸烯醇式丙酮酸羧激酶（PEPCK）ELISA試劑盒
雞ATP-檸檬酸裂解酶(ACLY)ELISA試劑盒
雞NADH脫氫酶ELISA試劑盒
雞ATP合酶（ATP）ELISA試劑盒
【關(guān)鍵詞】Hydroxycitric acid ? Acetyl-CoA ? ATP-citrate ? Energy metabolism ? Primary chicken hepatocytes
【DOI】 10.1159/000481564
【出版期刊】《CELLULAR PHYSIOLOGY AND BIOCHEMISTRY》
【產(chǎn)品原文引用】
Measurement of the key glucose metabolic enzyme protein content
Cells were cultured in 6-well plates (2×106 cells per well) ａnd treated with 0, 1, 10 or 50 μM (-)-HCA for 24 h. The cells were harvested ａnd disrupted ultrasonically in ice ａnd then centrifuged at 3000 rpm for 20 min at 4 °C. The supernatants were collected, the protein concentrations of glycogen phosphorylase (GP) (catalog #: E-75175), glycogen synthase (GS) (catalog #: E-75174), glucokinase (GK) (catalog #: E-76111), phosphofructokinase-1 (PFK-1) (catalog #: E-76131), pyruvate kinase (PK) (catalog #: E-76565), pyruvate dehydrogenase (PDH, E1) (catalog #: E-76118), citrate synthase (CS) (catalog #: E-75165), aconitase (ACO) (catalog #: E-75144) ａnd phosphoenolpyruvate carboxykinase (PEPCK) (catalog #: E-75117) were measured using ELISA kits according to the manufactures’ protocols (Shanghai Hengyuan Biological Technology Co., China). The enzyme protein content was normalized to total protein concentration in the sample.
原創(chuàng)作者：上海恒遠生物技術(shù)發(fā)展有限公司
總訪問量：9085228 管理登錄
